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| Uniformity Analysis of Watermelon (Citrullus lanatus) Varieties Amplified SSR Markers |
| LI Li1,Li Zhang2, 1, 1 |
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2. Beijing Official Seed Testing Station
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Abstract The goal of this research is to assess the availability of SSR markers for variety testing in watermelon hybrids and try to establish a standard for variety uniformity testing by SSR markers. A set of 11 primer pairs were applied for 78 watermelon (Citrullus lanatus) varieties and 97 watermelon hybrids (48 individuals per variety) of Beijing regional trial from 2009 to 2013, and one SSR marker per chromosome was selected. In accordance with the statistics of 175 watermelons testing results by 11 core primer pairs and different varieties specifics characteristic on different SSR locus, it was revealed that there were different individuals uniformity ratio on different SSR locus in one variety, the one individual uniformity ratio on one SSR locus did not represent the variety uniformity, and variety uniformity could be assessed not only based on individuals uniformity ratio on one SSR locus, but also individuals average uniformity ratio on 11 SSR loci. Comprehensively analyzing the statistics result of the 175 varieties uniformity ratio on 11 SSR loci illustrated that varieties uniformity ratio on SSR loci could be the standard-assisted for watermelon uniformity assessment. It was necessary to assess watermelon uniformity in combination with one individual uniformity ration on one SSR locus and average uniformity ratio on 11 loci, and there were 5 levels for evaluation, and explained the criterion evaluating watermelon variety uniformity by SSR markers and testing methods in details, preliminary draw up uniformity criterion of watermelon (Citrullus lanatus) varieties.
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Received: 29 September 2013
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Corresponding Authors:
LI Li
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