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Abstract Rice starch viscosity (RVA profile) characteristics can reflect the differences of starch quality of different varieties of rice sensitively, its genetic performance and the influence of starch synthesis-related genes(SSRGs) on RVA profile had received extensive attention. In this paper, we used backcross inbred lines as materials which were constructed by indica PTGMS line Guangzhan 63S and rice potential restorer line CG132R and composed of different allele combinations of soluble starch synthase Ⅱa gene (SSII-3) and granule-bound starch synthase gene (Wx) to study the influence of rice SSII-3 gene on RVA profile characteristics in non-glutinous rice, and compare the influence of SSII-3 gene with Wx gene on RVA profile characteristics. Main results were as follows:(1)gelatinization temperature had significant or extremely significant correlations with seven RVA profile characteristics except peak viscosity(PKV). It had significant or extremely significant negative correlations with hot paste viscosity(HPV), cool paste viscosity(CPV), consistence(CSV), setback(SBV) and peak time(PeT), but had extremely significant positive correlations with breakdown(BDV) and pasting temperature(PT). It indicated that SSII-3 gene had important influence on RVA profile characteristics in non-glutinous rice, and the influence being in the opposite direction compared with Wx gene. (2)Effect of SSII-3 gene on RVA profile characteristics was influenced because of the difference in Wx genotype. Split block design result showed that interaction between SSII-3 and Wx gene had significant or extremely significant influence on hot paste viscosity(HPV), cool paste viscosity(CPV) and consistence(CSV). When the Wx genotype of rice was TT(WxbWxb) or GT(WxaWxb), the consistence (CSV) of rice starch presented decreasing trend with the increase of gelatinization temperature, however, when Wx genotype of rice was GG(WxaWxa), consistence (CSV) had no regular variation trend. (3)Pasting temperature(PT) and peak time(PeT) of RVA profile characteristics were mainly affected by SSII-3 gene, however, peak viscosity(PKV), cool paste viscosity(CPV), hot paste viscosity(HPV), consistence(CSV), setback(SBV) and breakdown(BDV) were mainly affected by Wx gene. As the major gene controlling the gelatinization temperature of rice, SSII-3 gene is another important gene for RVA profile besides Wx gene, studying the influence of SSII-3 gene on RVA profile in non-glutinous rice and the interaction with Wx gene has important application value in evaluation of rice cooking and eating quality and breeding of high quality rice varieties.
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Received: 09 October 2013
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