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| Cloning of Homologous Auxin Receptor Genes in Cucumber (Cucumis sativus L.), and Analysis of Their Sequence Characteristics and Expression Patterns |
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Abstract Work had been done with the purpose to illustrate the regulation mechanism and function of auxin receptors and their signaling pathway in cucumber(Cucumis sativus L.), and also with the wish to find new approaches for enhancing production. Compared with the sequences of auxin receptor gene TIR1/AFBs in Arabidopsis, two highly conserved sequences were selected by screening the cucumber genome database, which were named CsTIR and CsAFB. cDNAs of both CsTIR and CsAFB genes were cloned and verified by sequencing. Overexpression vectors were constructed for further reverse genetic research in cucumber. Their sequences were submitted to GenBank and recruited with the accession of KC414935.1 and KC414934.1, respectively. The basic physical and chemical properties, as well as functional protein domains, of CsTIR and CsAFB were predicted by bioinformatics analysis. Phylogenetic and conservation analysis were taken between sequences from different plants, which made these two genes putative auxin receptors in cucumber. The expression patterns of the two genes were analyzed by semi-q RT-PCR. CsAFB and CsTIR both accumulated more in roots and leaves than in stems in 10-day-old plants while CsTIR was undetectable in stems. Expressions of both genes were detected in every tissue from 70-day-old flowering plants and showed no tissue specificity. This work provides new evidence of auxin receptors in cucumber, and will contribute to the further study of their functions and regulation mechanisms.
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Received: 16 April 2013
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