|
|
|
| Ochratoxin A-induced Cell Damage and DNA Hypomethylation in HepG2 Cell Line |
| 1, 1,韦露450103198306212562李丽婷120103198310162928 1, 1, 1 |
|
|
|
|
Abstract Ochratoxin A (OTA) is a mycotoxin contaminating food and feedstuffs. OTA causes nephrotoxic and hepatotoxic in experimental animals. In the present study, we investigated the oxidative damage and DNA damage induced by OTA. The results showed that OTA inhibited cell proliferation in a time and dose-independent manner, and increased the release of lactate dehydrogenase (LDH), glutamic-oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). We had also observed a dose-independent production of reactive oxygen species (ROS) and the decrease of superoxide dismutase (SOD) activity in HepG2 cells. OTA-induced DNA strand break, as detected by comet assay, was demonstrated in all group tested. Our work was the first time to research the effect of OTA on the global DNA methylation. The detection of 5-methyl-deoxycytidine (5mdC) indicated that OTA lowered the level of DNA methylation, but there was no significant difference among the groups. These results show that OTA induces oxidative damage, and disrupts the stability and integrity of DNA, which may be involved in OTA-induced hepatotoxicity.
|
|
Received: 22 October 2012
|
|
Corresponding Authors:
韦露450103198306212562李丽婷120103198310162928
|
|
|
|
|
|
|
