|
|
|
| Impact of Wounding or Agrobacterium tumefaciens GV3101 on Differential Transcription of Arabidopsis thaliana Inflorescence Stalk |
| 2, 2, 2, 2, 2, |
|
|
|
|
Abstract Gene expression or transcription profile of plant cells changes after inoculation with Agrobacterium tumefaciens. To understand the dynamic transcription and the modulation of metabolism pathways in plant infected with Agrobacterium tumefaciens, transcriptomic data of Arabidopsis thaliana inflorescence stalks from 3 h and 6 d after wounding or inoculation with GV3101 were analyzed by performing the statistical method RAM and the pathway analysis program MapMan. The results showed that 4 651 genes were differentially transcribed in the inflorescence stalks after inoculation with GV3101 while 3 885 genes changed in transcription after wounding, among which 1 045 genes were common. When inoculated with GV3101, the number of differently transcribed genes related to primary metabolism was reduced in comparison with the reference. Subcategorization of genes in stress category showed that 60 genes coding for PR proteins (pathogenesis-related proteins) were transcribed differentially after inoculation with GV3101, among which 37 genes coding for NB-LRR (nucleotide-binding site, leucine-rich repeat) disease resistance proteins were down-regulated. While in reference stalks, only 18 PR proteins-encoding genes were differentially transcribed, among which 6 genes coding for NB-LRR proteins were transcribed negatively. These results suggested that Agrobacterium GV3101 modulates gene transcriptional profile in Arabidopsis thaliana and suppresses disease resistance of host plant cells, which might facilitate the successful integration of T-DNA into host genome.
|
|
Received: 17 September 2012
|
|
|
|
|
|
|
