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| Polymorphism of the Exon 2 of Transforming Growth Factor-β1 Gene(TGF-β1) and Its Relationship with Litter Size in Two Goat Breeds |
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Abstract In order to find the molecular marker used in marker-assisted selection (MAS) to accelerate goat (Capra hircus) breeding progress, the goat transforming growth factor-β1 gene(TGF-β1) was selected to study the association between its polymorphism and litter size. According to the sequence of bovine TGF-β1 gene, three pairs of primers were designed to detect SNPs of intron 3 and exon 2 of TGF-β1 gene in two goat breeds by PCR-SSCP and DNA sequencing. The least square mean and genetic variance of different genotypes at polymorphic loci were analyzed. The results showed that the PCR products of primer 1 (P1) had polymorphism. Three genotypes (AB, BB, and AA), and one single nucleotide mutation (A→G) was detected in two goat breeds, and this mutation resulted in an amino acid change: Thr→Ala. Allele A was the dominant allele. The frequencies of allele A were 0.530 and 0.648 in two goat breeds, respectively. In Boer goat, AB genotype had significantly higher (P<0.05) litter size than that of BB and AA genotypes from the first to third parity. AB genotype was very significantly higher (P<0.01) than that of AA genotype, and significantly higher (P<0.05) than that of BB genotype, and BB genotype was significantly higher (P<0.05) than that of AA genotype in the fourth parity and average litter size. In Shaannan goat, AB genotype had significantly higher (P<0.05) litter size than that of AA and BB genotypes in the second, third, fourth and average parity, and AB and BB genotypes had significantly higher (P<0.05) litter size than that of AA genotype in the first parity. The TGF-β1 gene had significant effects on litter size in two goat breeds. Therefore, these results suggest that the TGF-β1 gene is a strong candidate gene that affects litter size in goats.
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Received: 16 July 2011
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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