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| Function Analysis of Putative Calmodulin(PCAM) and the Relationship between PCAM and Brassinosteroid(BR) Signal Transductionin |
| 1,wang fengru |
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Abstract To clarify the relationship between brassinosteroid(BR) and Ca2+ signal transduction and their regulating mechanism in plant growth and development, 2D-DIGE technology combined with mass spectrometry analysis was used to identify proteins differentially expressed in d61-4(BR insensitive mutant in rice(Oryza sativa L. ssp. Japanica)) and brd1-3(BR synthesis mutant in rice) by comparing with the wild type plant. A protein significantly upregulated both in d61-4 and brd1-3 was identified. The protein was related to Ca2+ signal transduction network(putative calmodulin, PCAM). This gene was overexpressed in Arabidopsis thaliana. Transgenic Arabidopsis lines overexpressing PCAM were smaller in size, and plant growth period was prolonged. The expression of SaurAC(a key gene in BR signal transduction pathway) in transgenic strains was obviously down-regulated. These results suggest a role of PCAM in the signal transduction of BR is as a negative regulator of the plant growth and development.
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Received: 07 March 2012
Published: 26 November 2018
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Corresponding Authors:
wang fengru
E-mail: wfr15931945160@126.com
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| [1] Nakamura A, Fujioka S, Sunohara H, Kamiya N, Hong Z, Inukai Y, Miura K, Takatsuto S, Yoshida S, Ueguchi-Tanaka M, Hasegawa Y, Kitano H, Matsuoka M The role of OsBRI1 and its homologous genes, OsBRL1 and OsBRL3, in rice. Plant Physiology. 2006, 140: 580-590[2] Reddy ASN..Calcium silver bulletin signaling. Plant Science. 2001, l60:381-404. [3] 关军锋，郑桂珍，李广敏. 渗透胁迫下钙对小麦胚芽鞘和根系生长的影响.中国农业科学，2005，38(3):486-491[4] Esealante.B.Z. and langille.A.R. Photoperiod, temperature, gibberellin，and antigibberellin affect tuberization of potato stem segments in vitro. Hortscience. 1998,33(4):701-703.[5] Balamani V.，Veluthambi，K. and Poovaiah，B.W. Effect of calcium on tuberization in Potato. PlantPhysiol.1986,80:856-858.[6] Yang T, Poovaiah B, W. Molecular and biochemical evidence for the involvement of calcium/calmodulin in auxin action. J Biology Chemistry: 2000, 3137-3143[7] Reddy AS, Reddy VS, Golovkin M. A calmodulin binding protein from Arabidopsis is induced by ethylene and contains a DNA-binding motif. Biochem. Biophys. Res. Commun.2000. 762-769[8] Yang T, Poovaiah B, W. An early ethylene up-regulated gene encoding a calmodulin-binding protein involved in plant senescence and death. J Biology Chemistry.2000. 38467-38473[9] Klahre U, Noguchi T, Fujioka S, Takatsuto S, Yokota T, Nomura T, Yoshida S, Chua NH. The Arabidopsis DIMINUTO/DWARF1 gene encodes a protein involved in steroid synthesis. Plant Cell.1998. 10: 1677-1690[10] Du Liqun, Poovaiah BW. Ca2+/calmodulin is critical for Brassinosteroid biosynthesis and plant growth. Nature 2005,437: 741-745[11] Nakamura A, Fujioka S, Sunohara H, Kamiya N, Hong Z, Inukai Y, Miura K, Takatsuto S, Yoshida S, Ueguchi-Tanaka M, Hasegawa Y, Kitano H, Matsuoka M. The role of OsBRI1 and its homologous genes, OsBRL1 and OsBRL3, in rice. Plant Physiology. 2006,140: 580-590[12] Hong Z, Ueguchi-Tanaka M, Fujioka S, Takatsuto S, Yoshida S, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M. The rice Brassinosteroid-deficient dwarf2 mutant, defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1, is rescued by the endogenously accumulated alternative bioactive Brassinosteroid, dolichosterone. Plant Cell. 2005,17: 2243-2254[13] Hong Z, Ueguchi-Tanaka M, Shimizu-Sato S, Inukai Y, Fujioka S, Shimada Y, Takatsuto S, Agetsuma M, Yoshida S, Watanabe Y, Uozu S, Kitano H, Ashikari M, Matsuoka M. Loss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organized arrangement and polar elongation of cells in the leaves and stem. Plant Journal. 2002,32: 495-508[14] Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M. A rice Brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell. 2003,15: 2900-2910[15] Hong Z, Ueguchi-Tanaka M, Uozu S, Agetsuma M, Ashikari M, Shimada Y, Fujioka S, Kitano H, Matsuoka M. Isolation and characterization of a rice brassinosteroid mutant, Osdwarf. Plant and Cell Physiology.2002, 43: 184-184 |
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