猪诱导多能干细胞(iPS)嵌合胚胎的制备

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Abstract Pig induced pluripotene stem cells(iPSCs) are ideal materials for regenerative medicine and cell biology research because of their self-renewal and multilineage differentiation potential. In order to explore the embryo chimeric ability of pig iPSCs, the piggyBac transposon vectors, PB[Act-RFP] DS and Act-PBase, were used to cotransfect pig iPSCs. The PS24-R, a pig iPS cell line, was successfully obtained which expressed red fluorescent protein (RFP). We have got chimeric embryos through microinjecting the PS24-R cells into the 4~8 cell parthenogenetic embryos in vitro. Then we collected the statistics of blastocyst rate and chimeric rate as the chimeric embryos developed to the blastocyst stage. The result showed that the PS24-R cell using piggyBac vectors to transfect the iPS cells could stably express RFP. The pig iPS cells could be effectively observed in chimeric embryos. We also tried to microinject one, five, ten PS24-R cells and one PS24-R clone respectively to pig embryos to produce chimeric embryos. Along with increasing of the number of donor cells, the chimeric rate of embryos gradually increased, whereas, blastocyst rate declined. Compared with the parthenogenetic embryos, the expression lever of the stem cell pluripotent factors, OCT4(octamer binding transcription factor 4), SOX2(sry related HMG box-2) and NANOG, had significant upregulated in chimeric embryos. In summary, the pig iPS marked by PiggyBac vector can achieve chimerism in the pig early embryonic development stage. This work lays a foundation for the production of pig iPS chimeric animals.

Key words： Porcine Induced pluripotene stem cell(iPSC) Parthenogenetic embryo PiggyBac transposon Chimeric embryo

Received: 01 May 2012 Published: 26 November 2018

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	Articles by authors
	GAO Xing
	MA Xiang-Xiang
	CHENG De
	MA Xiao-Ling
	YU Hua-Yan

Cite this article:

GAO Xing,MA Xiang-Xiang,CHENG De, et al. The Production of Pig Induced Pluripotene Stem Cell(iPSC) Chimeric Embryo[J]. , 2012, 20(12): 1349-1358.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2012/V20/I12/1349

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