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Abstract To characterize the structural protein VP gene of muscovy duck (Cairina moschata) origin Goose parvovirus (GPV) PT strain, a pair of primers were designed by DNAStar software based on the published sequences of goose original GPV and Muscovy duck parvovirus (MDPV) in GenBank. High fidelity PCR was performed, and the obtained PCR products were cloned into the pMD18-T vector, sequenced and analyzed. Sequencing analysis demonstrated that the VP gene of GPV PT strain included 2 199 bp encoding 732 amino acids (GenBank number: JF926695). The VP gene of GPV PT strain showed a combined feature of GPV and MDPV. It shared a nucleotide identity of 98.8% and an amino acid identity of 98.8% with muscovy duck origin GPV DY strain, which were much higher than the identities with that of goose origin GPV and MDPV. The VP1 gene unique part was similar to that of MDPV and the VP2 gene had the characteristic of goose origin GPV, which have not been reported previously. The success of this study has provided clues for the origin of muscovy duck origin GPV, and even make it possible to study the evolution and phylogenesis of Waterflow parvovirus.
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Received: 02 August 2011
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