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Abstract To identify the purity of hybrid rape exactly, steadily and effectively, this study has screened 3 of 479 pairs of SSR primers which could distinguish a chemical hybriding male varieties of hybrid rape Qinyou 33 from its parental lines effectively and steadily based on the establishment of efficient simple sequence repeat(SSR) agarose electrophoresis standard analysis system through the primer screening. The single-plant in three high pure lines was verified by using one of the primer SA332. Results demonstrated that the three single-plant SSR map had high consistency with its corresponding standard maps, reaching 98%, 99.33%and 96.67%, respectively. The practical detection and the consistency of qinyou33 commercial seed compared with single-plant growing in field using the primer SA332 and SA85 were carried out at the same time. The result showed that the consistency compared with single-plant grow in field reached above 96%, 98.67% at most and the consistency between the primer SA332 and SA85 reached 0.67% and 2.67% respectively. The purity deviation between 150 strains of indoor small groups and the field test more than 300 strains only reached -2.19% to 3.76%. With the basically consistency of indoor SSR tests result and field actual purity, it is fully demonstrated that the high consistency SSR correspondence analysis is a powerful tool for identify hybridism purity of rape especially for chemical hybiding male varieties of hybrid rape which are no significant difference in morphology.
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Received: 25 April 2011
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