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| Isolation and Culture of Porcine Skeletal Muscle Satellite Cells by Single Muscle Fiber and Their Muscle Differentiation |
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Abstract The present study established a porcine satellite cells in vitro model by means of the single fiber method to clarify the proliferation and myogenesis. Satellite cells can dissociate from the myofibers which isolated from porcine extensor digitorum longus (EDL) after digesting by collagenase Ⅰ. Then the cells were detected by their immunocytochemistry, growth curve, myogenesis induction and Western blot. The results showed that the cells adhered to the myofibers originally and then moved outside. More than 90 percent of the cells expressd the marker genes paired protein box(Pax7) and myogenic differentiation antigen(MyoD). After myogenesis induction, satellite cells began to convergence ,showed orientation growth, and finally formed multinucleated myotubule with expression of myogenin and myosin heavy chain(MyHC). Moreover, MyoD protein was highly expressed in proliferated stage, while the protein of myogenin and MyHC was detected only in the terminal differentiated stage.This study successfully cultured porcine satellite cells by means of the single fiber method and obtained high purity skeletal muscle satellite cells which can provide cell source for viable transplantation and cure related diseases.
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Received: 22 February 2011
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ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
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