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Abstract To investigate the transcriptional regulation of pig melanocortortin-4 receptor(MC4R) gene, 5' promoter fragment (spanned from -1 264 bp to -31 bp) was amplified with PCR method and the potential transcriptional start sites were predicted with bioinformatics method; Eleven promoter segments with different length were obtained by promoter deletion analysis and cloned into PGL3-Basic vector; then the promoter activities were determined in transiently transfected pig PK15 cell by the dual-luciferase assay system. The results indicated that there was a potential transcription start site in the region from intiation codon to -682 bp. Further analysis demonstrated that the region from -514 bp to -486 bp was important for the basic transcriptional activity and bioinformatics analysis showed that there was a cis-acting element possessing a functional binding site(HSF). HSF maybe play an important role in the regulation of MC4R gene expression.
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Received: 20 December 2010
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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