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Abstract Dehydrins is one of the responsive proteins under drought stress. The aim of this study was to explore distribution of dehydrins in cells and the relationship with degree of drought tolerance under water stress. Two different drought resistance winter wheat(Triticum aestivum) as experimental materials，with the colloidal gold immune electron microscopy technique and Western blot, ithe distribution of subcellular structures of dehydrated protein expression in wheat was nvestigated under water stress, and also analyzed its relationship with plant drought resistance was also analyzed. The results showed that: at the initial stage of the stress(4~8 h), the golden particles were mainly distributed in the cytoplasm, and also with a small amount in the nucleus. During the interim of the treatment(12~24 h), golden particles were mainly located in the cytoplasm and the nucleus, and also with a small amount in the organelle, at the end of the treatment(36~48 h), most golden particles were detected nearby the plasmalemma, the golden particles in the cytoplasm and the nucleus were relatively more after 24 h of the rehydration. Using the normal leaf tissue without any treatment and dehydrated protein antibody as contrast, however, none was detected. With the increase of stress time, the membrane permeability increased at beginning, the soluble protein content was also increased first; Western blotting analysis showed that ,there was a specific protein of 37 kD under water stress and its expression was positively correlated with wheat drought tolerance. After rewatering, relative permeability of cell membrane and soluble protein content were all decreased, and dehydrin could exist in plant for some time. In conclusion, these data provides a direct evidence for studying the expression and distribution of the dehydrated protein in wheat mesophyll cell.
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Received: 25 November 2010
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