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Abstract To better understand the transformation events of transgenic cottons in China and to develope the rapid and accurate identification methods, the integrated structure and genomic flanking sequence of transgenic cotton(Gossypium hirsutum L.) Ezamian 1, the new event of transgenic insect-resistant cotton in china were identified using the long distance PCR(LD-PCR) and the genome walking methods. The event-specific qualitative PCR method for Ezamian1 was established based on the flanking sequence through the optimization of primers, testing of the specificity and the sensitivity. The sensitivity of the method was 40 copies, corresponding to 0.1% in 100 ng template. Twenty one samples from the market was tested with this method, three samples were identified having the same event structure of the Ezamian1. All of these indicated that the event-specific detection method established this study may be useful for the identification of transgenic cotton in China.
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Received: 19 August 2010
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