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| SNPs Detection of Growth Hormone Gene (GH) in Qingyuan Partridge Chicken and Its Genetic Effects on Growth and Reproductive Traits |
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Abstract In order to investigate the association single nucleotide polymorphisms in GH gene with growth and reproductive traits in Qingyuan partridge chicken, the parallel typing system based on PCR-ligase detection reaction(LDG) was established in the present study, and used to detect the polymorphisms of G662A in intron 1 and T3094C and C3199T loci in intron 4 of growth hormone (GH) gene as well as its genetic effects on growth and reproductive traits in Qingyuan partridge chicken (Gallus gallus). The results were as follows: three loci exhibited polymorphisms and were coincidence with direct sequencing. There was no significant effect on growth and reproductive traits at locus G662A; At locus T3094C, individuals with TT genotype had significant older age at first egg and significant higher body weight at the age of 18 weeks than that of CC and CT genotype birds. At locus C3199T, TT genotype birds had significant higher average egg weight at the age of 50 weeks than that of CC and CT genotype birds. The combine genotypes also had significant genetic effects on average egg weight at first laying, average egg weight at the age of 50 weeks, and growth traits. However, it seemed that the effect of GH gene on growth traits was more significant; individuals with AACTCC combine genotype can be used as the molecular genetic marker to select Qingyuan partridge chicken for high body weight.
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Received: 21 June 2010
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YANG Han, ZHANG Yang-Hai, WANG Min, KANG Yu-Xin, ZHU Hai-Jing, LAN Xian-Yong, QU Lei, YAN Hai-Long, PAN Chuan-Ying. Polymorphisms of 3'-UTR of POU1F1 Gene and Its Association with Growth Traits in Shaanbei White Cashmere Goats (Capra hircus)[J]. 农业生物技术学报, 2019, 27(7): 1224-1232. |
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