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Abstract Banded leaf and sheath blight (BLSB) becomes an emerging worldwide problem in maize production, which caused by the fungal pathogen Rhizoctonia solani Kühn. High-resistance lines R15 and susceptible lines Ye478 were selected as materials to elucidate the expression mechanisms of protein in maize leaves infected by R. solani Kühn, Total proteins were extracted from inoculated leaves during different times(0, 12, 24, 36 and 48 h) respectively and then analyzed by IEF/SDS-PAGE gel electrophoresis technique. Eight hundred protein spots were obtained, and of which, several proteins showed differences in expression levels and fifteen different protein spots were selected to analysis by ionization flight time mass spectrum facilitated by matrix auxiliary laser, and combining with the bioinformatics analysis and on-line database. It is showed that several differential proteins were induced by R. solani Kühn in different maize inbred lines. Of which, Shikimate kinase Ⅰ, Ferredoxin- thioredoxin reductase catalytic chain (FTR) increased their expressions which induced the resistance system startup, while photo-systemⅠreaction center subunit N, Ribulose-bisphosphate arboxylase/oxygenase large subunit ruduced their expressions which ihibited the material and energy metabolism, and then made the susceptible plants death.
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Received: 17 May 2010
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