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Abstract Soybean cyst nematode (SCN) is a pest which can cause worldwide soybean yield losses seriously. The resistance of soybean to SCN is controlled by multiple genes. It is very important to detect the disease-resistant genes and breed the SCN disease-resistant cultivar. In order to understand the relationship between the gene and resistance to SCN, we analyzed the expression characteristics of candidate gene GmHslpro-1 on Soybean (Glycine max) resistant line Zhongpin03-5373 and susceptible cultivar Zhonghuang 13 using the Real-time PCR technique. We found that the relative expression of the gene raised after 1 d vaccinating SCN 4 Race (SCN 4) and it was 13.4 times higher than that of the control sample, it kept upward until inoculated 6 d. Analysis of variance showed that the relative expression of the gene was significant difference between inoculated and un-inoculated at significant difference (0.05 or 0.01 level). It indicates that GmHslpro-1 may be induced to express in the conditions of SCN 4 infection, and the gene has a certain resistance to SCN 4.
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Received: 16 April 2010
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