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Abstract Pigeon is a kind of monomorphic bird, and also a typical monogamous bird, this situation challenges the couple and human-assisted breeding of pigeon(Columbalivia Gmelin). Using feathers of pigeon as DNA sources , a PCR test was used to amplify two homologous fragments of the CHD-W(380 bp) gene, unique in female, and the CHD-Z(450 bp) gene, occurring in the two sexes. The results showed that high-quality genomic DNA was extracted from feathers of pigeon, and there was a single CHD-Z band in males but females had both, CHD-Z and CHD-W band. This sexing technique is objective and non-invasive and can be useful in the management of pigeon production.
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Received: 08 September 2009
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ZHAO Zhong-Hai, ZHOU Di, LI Hui, YI Heng-Jie, LONG Qing-Meng, SHI Xin-Cai, BU Xiao-Yan, Chen Lin, YANG Hua-Ting, LI Xing-Cai. Genetic Diversity and Origin of Guizhou Pig (Sus scrofa domesticus) Breeds Analyzed by mtDNA D-loop Region[J]. 农业生物技术学报, 2019, 27(7): 1206-1214. |
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