Abstract Pigeon is a kind of monomorphic bird, and also a typical monogamous bird, this situation challenges the couple and human-assisted breeding of pigeon(Columbalivia Gmelin). Using feathers of pigeon as DNA sources , a PCR test was used to amplify two homologous fragments of the CHD-W(380 bp) gene, unique in female, and the CHD-Z(450 bp) gene, occurring in the two sexes. The results showed that high-quality genomic DNA was extracted from feathers of pigeon, and there was a single CHD-Z band in males but females had both, CHD-Z and CHD-W band. This sexing technique is objective and non-invasive and can be useful in the management of pigeon production.
