Abstract BALB/c mice were immunized by purified Bovine viral diarrhea virus-1（BVDV1）and Bovine viral diarrhea virus-2 （BVDV2）. Murine myeloma SP2/0 cells were fused with the splenocytes of the immunized mice. Two indirect ELISA with BVDV1 and BVDV2 as coated antigen separately were used to screen antibody-producing hybridomas. Three McAbs against BVDV were obtained, one of them showed positive reaction only to BVDV1 named BV1; another showed positive reaction only to BVDV2 named BV2; the third one could react with both BVDV1 and BVDV2 named BV12. Cross reaction tests suggested that both BV1 and BV2 were specific to BVDV1 and BVDV2 respectively, BV12 was also reactive with Hog chorera virus (HCV) besides BVDV1 and BVDV2. The ascites titers of BV1、BV2、BV12 were 105、106、105 respectively, and all of them had neutralization activity, the highest neutralization titer was 1：741455. The subtypes of BV1、BV2、BV12 were IgG1、IgG2a、IgG2a respectively, the light chain of all McAbs was ????chain. The chromosome configuration of the three hybridoma cells were102、99、100 respectively.
