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| Acquirement of the Transgenic Maize Harboring Bt cry1Ah Gene and Analysis of Its Inheritable Stability |
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Abstract The cry1Ah gene is one of the novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. A plant expression vector pHUAh harboring the cry1Ah gene was constructed and transferred into maize (Zea mays L.) by microprojectile bombardment. Thirteen regenerated plants were PCR positive. Bioassay results showed that events B1-1 and B1-7 had high resistant to the Asian corn borer (Ostrinia furnacalis). These two events were analyzed for 4 years from T1 toT5 generations. The results showed that foreign gene cry1Ah was expressed stably in maize and could be inherited stably over generations. Cry1Ah protein expressed in events B1-1 and B1-7 and their different tissues were analyzed by ELISA. The results exhibited that the contents of Cry1Ah protein were different between the events B1-1 and B1-7, as well as among different tissues. Results of bioassay to T2~T5 transgenic maize plants suggested that the transgenic plants had high resistance to the Asian corn borer and the resistance can be inherited stably. So events B1-1 and B1-7 can be potential candidates for the breeding of Bt insect-resistant transgenic maize.
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Received: 31 March 2009
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