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Abstract BALB/c mice(Mus musculus) were immunized five times with purified recombinant rGST-BoIFN-γ protein expressed in E.coli BL21(DE3) at 100μg per mice. Murine myeloma cells were fused with splenocytes of the immunized mice. An indirect ELISA with recombinant rHIS-BoIFN-γas antigen was used to screen antibody-producing hybridomas. Two hybridomas cells could produce McAbs steadily after 3 cycles of subcloning, all McAbs showed positive reaction to rHIS-BoIFN-γ in western blot. All McAbs were IgG1 isotype and κ chain. The two McAbs can be partner for detect antigen.
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Received: 09 April 2009
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