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Abstract Abtstact：The elimination of marker genes after selection is a main premise for the commercial use of genetically modified plants, and site-specific recombination can be used to eliminate maker gene. There are three main site-specific recombination systems which can be used successfully in plants: Cre/loxP, FLP/FRT and R/Rs. To remove selectable marker genes from transgenic plants by the site-specific recombination system, there are generally several strategies: constitutive expression of recombinase by re-transformation of Cre gene or crossing with Cre transgenic plant, or application of transient, inducible or tissue-specific expression of recombinase, among which inducible autoexcision is the most potential way in plant transformation.
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Received: 13 May 2009
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