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PCR-RAPD molecular sub-typing and virulence genes detection of Campylobacter jejuni isolated from eastern China
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Abstract  C. jejuni isolated from different sources (n=113) were subtyped by PCR-RAPD. The PCR products showed 15 different banding profiles, In this study, V7、V9、V10、V11、V12、V13、V14、V15, inhabited 81.42% profile numberV10、V11、V12 and V14 were predominant in the isolates.Which were 11.50%、12.39%、13.27% and 10.62%.But C1-C5、V6、V8 were lower than 1%.Besides, isolated from different sources with V7、V10、V11、V12、V14.It showed that isolates from different sources with a high degree of cross-distribution. Of the 113 C. jejuni isolates tested, the average positive rates of the adhesion related gene cadF, racR were 92.45% and 38.69%, the flagellar gene flaA was 73.58%, but the average rates of the virulence-associated genes cdtA、cdtB、cdtC、wlaN and virB11 were 71.70%、52.83%、96.23%、12.26% and 1.89%. Average rates of the heat shock protein and transporter associated protein gene dnaJ、ceuE were0.94%和65.09%,Average rates of gene CiaB、pldA were 39.62% and 9.43%,58.82% strains carried over 6 virulence-associated genes.
Key wordsCampylobacter Jejuni      molecular typing      RAPD-PCR      virulence-associated genes     
Received: 24 July 2009     
Corresponding Authors: Jiang Yuan   
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NULL. PCR-RAPD molecular sub-typing and virulence genes detection of Campylobacter jejuni isolated from eastern China[J]. , 2010, 18(3): 586-591.
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http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2010/V18/I3/586
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