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Abstract According to the highly conservative region of Hormone-sensitive Lipase (HSL) gene sequences from bovine (Bos taurus), human(Homo sapiens) and mouse (Mus musculus) in the GenBank, the specific primers were designed to clone the CDS region of HSL gene in mammary gland of Xinong Saanen dairy goats using the RT-PCR method, the bioinformatic analyses of nucleotide sequence, the deduced amino acid sequence, and the prediction of protein structures of HSL gene were conducted. The results showed that the full length of CDS region of HSL gene was 2271 bp encoding 756 amino acids (GenBank accession NO. EU273879), homogeneities of CDS nucleotide sequence between goat and bovine, human and mouse were 96%, 86%, and 79%; that of amino acid sequence were 96%, 85%, and 83%, respectively, the predicted molecular weight and isoelectric point of HSL gene were 82583.7 Da and 6.23, the similar structure of CDS region of HSL gene indicated the possible functions of HSL gene in milk secretion.
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Received: 24 March 2009
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Corresponding Authors:
Jun LUO
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