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| The optimization of fermentation process to increase purity of thuringiensin in the extract from supernatant liquid |
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Abstract Abstract: B. thuringiensis subsp.chinensis strain CT-43 highly produces thuringiensin (Thu). Meanwhile, other metabolizing product (ZZ) was synthesized, but was difficultly removed from Thu. In this study, various media were screened and the G medium was found to inhibite the ZZ synthesis. Based on the G medium, feed-batch fermentation and orthogonal arrays was combined to further inhibit ZZ synthesis and increase the yield of Thu.. The optimal fermentation process was that, After strain CT-43 was cultured in G medium for 20h, patching with 2.0 g/L sodium citrate and 16 g/L glucose and culturing for additional 32h, the peak area ratio of Thu could get 74.84 in the primary extract, which was 2.5 times for 29.85% of LB fermentation. These onditions could create a favorable fermention process to produce more pure Thu and it will enefit for further research. Lastly, HPLC-MS was used to verify the produced Thu. Thu became he major element during the feed-batch fermentation. No ZZ was detected and Thu was not modified after above fermentation process.
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Received: 22 April 2009
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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