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| Cloning, expression and activity analysis of zearalenone-degraded enzyme |
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Abstract Zearalenone (ZEN) was the most widely distributed Fusarium toxins all over the world. ZEN could lead to a series of estrogenic effects symptoms such as infertility and abortion , damage the internal organs, and even promote the cancer cells to grow.. Over the past decade, the researches on ZEN degradation had made some progress and a gene zhd101 encoded lactose hydrolase that possessed the capability to transform ZEN into non-toxic products(including plant cells) had been reported in Japan. In our research, a DNA fragment (ZEN-jjm ) was obtained by coloning from Gliocladium roseum, and there were 9 different bases in this sequence contrasting to the gene zhd101. Subsequently, ZEN-jjm was expressed through prokaryotic host, and the results indicated that the expressed protein could degrade ZEN significantly.
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Received: 03 September 2009
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