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| Isolation and Functional Characterization of GLABRA2 Promoter Related to Trichome Development in Brassica napus |
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Abstract GL2 gene plays key roles in trichome development of Arabidopsis. A putative GL2 promoter was isolated from genomic DNA of Brassica napus by genomic walking method. Bioinformatics analysis indicated GL2 promoter has low homology between Arabidopsis and B. napus. To study the function of this promoter, a GP1-GUS construct was made and introduced into Arabidopsis by Agrobacterium-mediated transformation method. Ten positive transformants were obtained. Segregation analysis in T2 generation revealed that each of six lines segregated as 3:1 for kanamycin-resistance, suggesting T-DNA insertion in a single copy. Histochemical staining analysis showed that the GUS gene mainly expressed in young tissues of Arabidopsis, such as cotyledons, trichomes of true leaves and roots, which are basically consistent with the expression style of Arabidopsis.GL2 promoter. However, compared with Arabidopsis GL2 promoter expressed in the whole processes of trichome development, B. napus GP1 only expressed in the early stage after the trichome initiation of Arabidopsis.
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Received: 16 January 2009
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Corresponding Authors:
Liu sheng yi
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