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Abstract Preadipocytes were obtained from newborn Chinese Holstein calves and induced differentiation for 9 days. The cells became larger and circular accompanied by the small lipid droplets confluenting into large ones and increased mRNA expression of PPAR-γ. At same time ,the expression of Pref-1 was decreased and cells differentiated ultimately into typical adipocytes. Using semi-quantitative RT-PCR method, 1 nmol/L insulin did not affect the expression adiponectin mRNA, but 10, 100 and 1000 nmol/L insulin decreased expression adiponectin mRNA 48.7%,61.4% and 61.9%, respectively (P<0.05). Meanwhile, 100 nmol/L insulin in time-dependent fashion inhibits expression adiponectin mRNA within 24 h. Furthermore, inhibition of PI3K/Akt with LY294002 reverses the inhibitory effect of insulin on adiponectin mRNA expression. These results suggest that insulin at least partially suppressed the expression of Adiponectin mRNA via the PI3K/Akt pathway.
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Received: 02 March 2009
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ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
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