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Abstract Abstract: The objective was to determine the effect of estrous cow serum on porcine oocyte maturation. In the experiment, various sera was added to maturation medium and observed matured results. The effect of serum to oocyte quality was tested by in vitro developmental abilities of parthenactivation and reconstructed embryo. Moreover, in vivo development ability of nuclear transfer embryos constructed by oocytes matured with medium supplemented estrous cow serum was tested. Results indicated that there was no significant difference in rate of maturation among three sera (estrous cow serum, cow serum, and fetal cow serum) supplementation medium (P>0.05). In vitro developmental capacities of oocyte matured in medium supplemented various sera were not significantly different (P>0.05). Embryos reconstructed with fetal fibroblast cell and oocyte derived estrous cow serum medium completed full-term development.
Key words: porcine; oocyte; in vitro maturation; estrous cow serum; parthenactivation; nuclear transfer
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Received: 18 February 2009
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