Abstract The objective of this experiment was to establish an effective JIVET technology system on prepubertal dairy Cattle. In this experiment, we set up an effective gonadotropic hormone stimulating protocol on calf, and 31 oocytes per calf on average were obtained. After in vitro maturation and in vitro fertilization, the fertilization rate of calf oocytes reached 63.2%, which was no significant difference from matured bovine oocytes (59.7%). After embryo in vitro culture, the blastocyst rate of calf embryos was 31.6%, which was still significantly lower than matured bovine embryos (48.0%). After embryos transferred to 3 recipients, 2 of them became pregnant, and 1 recipient completed the whole course of pregnancy.
