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Abstract Lycopene, one kind of pigment carotenoids, performing the important function on antioxidant, disease prevention and so on. Lycopene cyclase-β(Lyc-β) is one of the key enzyme in the carotenoid biosynthesis pathway. Two fragment of 300 bp from Lyc-β gene were reverse transcribed and amplified from tomoto mRNA according to Genbank sequence X86452. One intron fragment of 170 bp was amplified from gusA gene. Two plant expression vectors to interference Lyc-β gene were constructed by ligation the intron fragment of gusA gene with two Lyc-β fragments which were designed in different direction separately. After transformation into Lycopersicun esculentum through Agrobacterium tumefaciems, the interference vectors were used to analyses the interference effect. The twenty-two transgenic tomato plant were obtained to perform real time PCR assay. The results demonstrated the different interference efficacy to the mRNA abundance of Lyc-β gene in average of 11.78% and 13.86% separately Compared with the control of wild plant. The lycopene content of transgenic tomoto were analyzed by HPLC, and its highest content was 13.84 μg/g FW, 4.26 fold compared with control.
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Received: 29 March 2009
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