|
|
|
Abstract Five pairs of primers (P1-P5) were designed to detect single nucleotide polymorphism of neuropeptide Y-Y1 receptor (NPY-Y1R) gene in sexual precocity and high fecundity breed (Jining Grey goat) and sexual precocity and moderate fecundity breed (Boer goat) and sexual late-maturing and low fecundity breeds (Angora and Inner Mongolia Cashmere goats) by PCR-RFLP and PCR-SSCP. The results showed that only the products amplified by primers P2, P4 and P5 displayed polymorphisms. As for P2, three genotypes (AA, AB and BB) were detected in Jining Grey, Inner Mongolia Cashmere and Angora goats, only two genotypes (AA and AB) were in Boer goats. Sequencing revealed one mutation (C625G) in genotype BB compared with genotype AA, which caused no amino acid change. No significant difference (P>0.05) was found in litter size between AA, AB and BB genotypes in Jining Grey goats. As for P4, two genotypes (CC and CD) were detected in four goat breeds. Sequencing revealed one mutation (G1141A) in genotype CD compared with genotype CC, which caused no amino acid change. No significant difference (P>0.05) was found in litter size between CC and CD genotypes in Jining Grey goats. As for P5, two genotypes (EE and EF) were detected in Jining Grey goats, only one genotype EE was in other three goat breeds. Sequencing revealed one mutation (C1330T) in genotype EF compared with genotype EE, which caused no amino acid change. The differences of genotype distribution were highly significant (P<0.01) between Jining Grey goats and three late-maturing breeds, and no significant difference (P>0.05) was found between three late-maturing breeds. The Jining Grey goat does with genotype EF had 0.58 (P<0.01) kids more than those with genotype EE. These results preliminarily indicated that F allele of NPY-YIR gene was associated with sexual precocity and prolificacy in Jining Grey goats.
|
|
Received: 21 January 2009
|
|
|
|
| [1] |
ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
|
|
|
