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Abstract Bacillus TS01 is a functional strain separated by our laboratory. TS01 strain and other 15 bacillus type strains (B. Licheniformis, B. Subtilis, B. Pumilus, B. megaterium , B. Coagulans, B. cereus , B. lentus , B. thuringiensis , B. thermoaerophilus , B. amyloliquefaciens , B. circulans ,B. sphaericus , B. laterosporus , P. polymyxa , V. Pantothenticus.) were used to be differentiated with amplified ribosomal DNA restriction analysis (ARDRA). 16S rDNA was amplified by the general primer pair 16S-27and 16S-1525. The 16S rDNA PCR amplicon (about 1.5 kb) was digested by six restriction enzymes (Alu I,Taq I,Mse I,Bst UI,Hha I and Tsp509 I ), The characteristic ARDRA fingerprint of TS01 was obtained. The ARDRA patterns were analyzed by GelcomparⅡ software using UPGMA method. The homology tree based on ARDRA analysis shows that TS01 strain belongs to Bacillus licheniformis, this result is identical to the identification results before in our lab by using morphology, biochemistry and 16S rDNA sequence analysis to identify TS01, which shows that the established technique of ARDRA analysis has the dependability for the identification of TS01 strain on the specific level.
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Received: 13 December 2008
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