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Research of Genetically Modified Maize Detection with Multiplex PCR-Gene Chip
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Abstract  Based on integrated DNAs construct of seven genetically modified maize, Bt11, Bt176, Mon810 , Mon863, TC1507, GA21 and NK603,event specific primers were designed for polymerase chain reaction .In addition, event specific oligo probes were constructed by analyzing integrated DNAs structure of genetically modified maize. All the event specific probes could be completed in one microarray . The results indicate that the sensitivity of oligonucletide microarray (0.01%) was higher than that of the electrophoresis method (0.1%),meanwhile the detection efficiency was highly improved by using multiplex PCR.
Received: 13 October 2008     
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NULL. Research of Genetically Modified Maize Detection with Multiplex PCR-Gene Chip[J]. , 2009, 17(6): 1075-1082.
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http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2009/V17/I6/1075
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