Molecular cloning and sequence analysis of a new β-1, 4-endoglucanase gene(Dd-eng-1b)from migratory plant-parasitic nematode Ditylenchus destructor on sweetpotato in China
Abstract Endoglucanase secretions from the oesophageal glands of plant-parasitic nematodes have played important roles in invasive plant.. A new β-1, 4-endoglucanase gene, named Dd-eng-1b,was isolated from Ditylenchus destructor,by RT-PCR and RACE. the full length cDNA of Dd-eng-1b consists of 1640 bp, with a 1443 bp ORF encoding a 481 amino acid protein (GenBa nk acession No. FJ430142) . The putative protein with theoretical molecular weight is 50.89kD and pI 6.94. The sequenceing analysis indicated that this gene is classified as a member of Cellulase (glycosyl hydrolase family 5, GHF5) and has a 19 amino acids long singal sequence at N terminal and a high similarity to a bacterial type cellulose-binding domain Ⅱ at C terminal
Comparative analysis between cDNA and Genomic showed that Dd-eng-1b contain four introns，demarcated by 5'-GT...AG-3' in the slpice sites，and phylogenic analysis suggested that Dd-eng-1 had a strong homology to Bacillus.subtilis and Erwina carotovora, which may be indicative of an ancient horizontal gene transfer from bacteria.
De-liang Peng,Huan Peng. Molecular cloning and sequence analysis of a new β-1, 4-endoglucanase gene(Dd-eng-1b)from migratory plant-parasitic nematode Ditylenchus destructor on sweetpotato in China[J]. , 2009, 17(6): 1035-1041.
