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Abstract In order to analyse gene expression pattern in the sugarcane leaves and set the basis for the cloning of some important functional gene, a full-length cDNA library was constructed by the method of Oligo-capping, taken functional leaves from three development stage of sugarcane as plant material. After identification, the titer of the library was 1.8×105pfu/mL, the recombinant rate was 89%, the average size of inserted fragment was about1.0kb. In total, 228 efficient EST(expressed sequence tags) of 5’ end were obtained from this cDNA library. bioinformatics analysis showed these ESTs were assembled into 154 TUTs(tentative unique transcript, TUT),The homologous analysis of the 154 TUTs on NCBI showed that these 64 TUTs (including 94 ESTs) were determined to be the tags of gene with putative function, these genes were found to be involved in the biological process including cell development, signal transport ,protein synthesis, transcription, stress tolerance response, energy metabolism and so on.
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Received: 21 November 2008
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