|
|
|
Abstract Late embryogenesis abundant (LEA) protein gene is an important gene in plant stress response. In this paper, a full-length cDNA sequence of sugarcane Lea gene was obtained from sugarcane leaf full-length cDNA library through sequencing and validated by the corresponding bioinformatics analysis , termed Sc-Lea . The full-length of Sc-Lea is 921 bp, with a 495 bp open reading frame(ORF), with a 64 bp 5’ untranslated region(UTR) and 362 bp 3’ UTR . At the 3’ end UTR, it has a clear polyA structure, the sequence around the start codon is CCCCAGCCATGGC,and the base of -3、+4 is G and C respectively which is line with the rules of Kozak. Then, the prokaryotic expression vector, which contained the ORF of sugarcane Lea gene, was constructed and the targeted protein at the molecular weight 17.9kD coding by sugarcane Lea gene was induced by IPTG. Moreover, in order to explore the expression characters of sugarcane Lea gene under the stress of SA, H2O2, PEG and NaCl, the Real-time qPCR approach was applied. The results showed that the expression of the sugarcane Lea gene was induced both by PEG and NaCl, while inhibited by H2O2. Also, the expression of this gene could be influenced by SA. It could be to some extent inferred that the sugarcane Lea gene obtained in this study plays an important role in the sugarcane drought-tolerant and salt-tolerant mechanism.
|
|
Received: 20 November 2008
|
|
|
|
|
|
