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Abstract The Tianzhu White Yak’s SRY gene was amplified from Yak’s genome. The product was cloned to pGEM-T easy vector and sequenced by biological company. The total coding region of Tianzhu White Yak’s SRY gene is 687 bp, encoding a peptide with 229 amino acid residues. Through sequences aligned, it was discovered that there are 2 mutations in the coding region between yak and cow, causing 1 amino acid mutation in LF protein. The coding region of Yak’s SRY gene was cloned to EcoR I and Sal I sites of pET-28a(+) vector to construct an expression plasmid pET-28a/SRY. The expression plasmid was transformed to E.Coli BL21 (DE3), and induced under proper conditions then the SRY protein was highly expressed. The product of expression was identified by Western-bloting to be sure that the SRY protein of yak was expressed.
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Received: 15 August 2000
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