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Abstract The single nucleotide polymorphisms(SNPs) within the coding regions of CXCR1 gene in Holstein cattle and Luxi Yellow cattle and Bohai Black cattle were investigated by nested-PCR, DNA sequencing technology and CRS-PCR method. Sequencing results showed that three SNPs were identified, which they were 819(G/A),995(G/A),1008(C/T). 819(G/A) and 1008(C/T) were nonsense mutations, but 995(G/A) resulted amino acids alteration Arg422His.In Holstein cattle and Luxi Yellow cattle and Bohai Black cattle, G and G and T predominan allele were identical at 819(G/A) and 995(G/A) and 1008(C/T), which were G , G , T and the allelic frequencies were 0.63/0.73/0.71, 0.60/0.85/0.71, 0.74/0.76/0.71 respectivly. Chi-square test indicated that 995(G/A)and 1008(C/T) of Luxi Yellow cattle and all the sites of Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium (P>0.05), while all polymorphic sites were not meet Hardy-Weinberg equilibrium (P<0.05) in Chinese Holstein cattle. The value of polymorphism information content indicated that 819(G/A) and 1008(C/T)s were moderate polymorphism in three breeds（0.25＜PIC＜0.5）, while 995(G/A) were slight polymorphism in Luxi Yellow cattle and were moderate polymorphism in other two breeds.
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Received: 08 August 2000
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