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| Cloning and splicing expression in Escherchia coli |
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Abstract Getting the chitinase catalytic domain of Streptomyces roseoflavus by PCR, The catalytic domain was then connected with S. lividans chiC fragment which contains three domains (cellulose binding domain, chitin-binding domain, signal peptide domain). The fragment was cloned into pET23b(+). Then the recombinant plasmid pLCH was transformed into E. coli JM109(DE3). The transformat was induced expressing with IPTG. Its chitinase activity was be found on the chitin culture medium.
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Received: 28 March 2006
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