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| Ontogenetic Expression of SGLT1 and GLUT2 mRNA in Duodenum of Different Genotype Broiler Chicken |
| WANG Xiu-qi;TAN Hui-ze;SHU Gang;SU Hai-lin;DAI Fa-wen;FENG Ding-yuan |
College of Animal Science, South China Agricultural University, Guangzhou 510642, China
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Abstract In order to investigate the ontogenetic expression of sodium/glucose cotransporter1(SGLT1) and glucose transporter2 (GLUT2) mRNA in duodenum of different genotype broiler chickens, 120 1-day-old parental male Arbor Acre (AA) broiler chickens and 120 parental male yellow cover chicks were randomly divided into 4 replicates, respectively. The productive performance was recorded from day 1 to 58 d. On 2,16,30,44 and 58 d, duodenum samples were collected. The mRNA expression abundances of SGLT1 and GLUT2 at different developmental stage were determined by relative quantitative RT-PCR. It was found that: (1) The average daily feed intake (ADFI) and average daily gain (ADG) of every week of AA chicken were higher than that of yellow cover chicken (P <0.05) , the change curve of every week ADFI and ADG were similar between the two genotypes. (2) Ontogenetic expression model of SGLT1 mRNA were consistent between AA broiler and yellow cover chicken. The SGLT1 mRNA abundance in the two genotypes were increased from day 2 to day 30, then decreased on day 44 and increased again on day 58. The SGLT1 mRNA expression level of AA chicken was higher than that of yellow cover chicken and the differences were significant on day 16 and 30 (P<0.05). (3) GLUT2 mRNA expression of the two genotypes trended to increase from day 2 to day 30, and the abundance of day 16 and 30 were higher than that of day 2 at significant level (P <0.05). On day 44 and day 58, the GLUT2 mRNA expression of AA broiler were lower than that on day 16 and 30 (P <0.05), while the expression of yellow cover chicken was going on increasing from day 44 to day 58 and GLUT2 mRNA expression abundance of day 58 was higher than that on day 2,16 and 30; Compared GLUT2 mRNA expression with yellow cover chicken, AA broiler was higher on day 16 and 30 (P <0.05), while on day 58 yellow cover chicken GLUT2 mRNA expression was higher than that of AA chicken. These results demonstrate that: 1) Productive performance of AA broiler chicken excells yellow cover chicken. The alteration of nutritional level has great effect on the performance and the AA broiler chicken is more sensitive than yellow cover chicken. 2) SGLT1 mRNA expression of the two genotype has the same ontogenetic model in duodenum and paralleled with the productive performance. To change diet can decrease the expression of SGLT1 mRNA, indicating that the productive performance can be improved by up-regulating of SGLT1 expression. 3) GLUT2 mRNA expression ontogenetic model of late-finishing yellow cover chicken is distinct with AA chicken, and its mechanism and effect on production needed further study.
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Received: 20 September 2005
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Corresponding Authors:
FENG Ding-yuan
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