Effect of Buffer Systems on Elastase Production by Bacillus sp. EL31410
XU Ying1 HE Guo-Qing1* CHEN Qi-He1 LI Jing-Jun2
(1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; 2. School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China)
Abstract Abstract：The elastase is a kind of protease that can hydrolyse elastin. pH is one of the most important environmental factors for cell growth and product accumulation. The best pH for cell growth and elastase production were about 6.0～7.0 and 6.4～6.6, respectively. Single factor and rotatable orthogonal central composite design were used to study the effect of buffer systems on producing elastase. The optimal result showed that 15 h later adding pH 6.4 (0.02 mol/L KH2PO4-NaOH) buffer to medium was the best way for cell growth and elastase production. This model was verified to be credible.
