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Detection of a New Virulence Associated Gene in Streptococcus suis
Type 2 Jiangsu Isolate |
| Li Ganwu Yao Huochun Lu Chengping** |
| (College of Veterinary Medicine, Nanjing Agriculture University, Jiangsu 210095, China) |
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Abstract Abstract: Streptococcus suis type 2 is an important pathogen for pig and human. It has different virulence factors including muramidase-released protein(MRP), extracellular factor(EF), suilysin and capsular polysaccharide. Smith H E et al(2001) had successfully detected a new virulence associated sequence orf2 by using the method in vivo complementation. It was interesting to investigate the new orf2 in swine streptococcal strains isolated in China. The results showed that the new orf2 was detected from genomic DNA of Streptococcus suis type 2 Jiangsu isolate HA9801 by polymerase chain reaction(PCR) technique and the PCR product from isolate HA9801 was choned into plasmid vector pET-32a(+) via restriction endonuclease Bam HⅠand Hind Ⅲ. The recombinant fragment was verified by restriction endonuclease analysis and sequenced. The nucleotide sequence was 100% homological to that of the new hypothetical virulence assolated sequence orf2 reported by Smith H E. By the same method, this orf2 was also detected from genomic DNA of Streptococcus suis type 2 strains and other streptococcal isolates from swine in China. 7/8 of Streptococcus suis type 2 isolates and 9/23 of other streptococcal isolates from pigs were positive. The positive ratio of the orf2 in all isolates including Lancefield group A, B, C, D, R and other serotypes of Streptococcus was 51.6%(16/31). It suggested that the new orf2 distributed widely among streptococcal strains of pigs from different areas in China. The pathogenicity of the orf2 would be further studied
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Received: 01 January 1900
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Corresponding Authors:
Lu Chengping**
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