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| Analysis of the Secreted Materials from Epithelial Cells Cultured on Mantle of Pearl Oyster（Pinctada martensii） |
| Wang Aimin1 Yan Bing2 Su Qiong2 Ye Li2 Chen Wenguang2 |
| （1. Ocean College, Hainan University,Haikou 570228,China; 2. Guangxi Institute of Oceanography, Beihai 536000,China) |
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Abstract Abstract: Using the tissue culture technique, the mantle of the pearl oyster (Pinctada martensii ) in vitro was cultured.The epithelial cells migrated out from mantle kept activity and function of the secretion. The secreted materials (SMs) were studied by polarizing microscope and scanning electron microscope. After 4-day culturing， round epithelial cells changed in shape, and began to secrete fine granules. During culture, the epithelial cells secreted increasingly，and the SMs became large granules. The birefringence were show under the polarizing microscope. After 34-day culture, the SMs accumulated into a mass of granules, appearing strong birefringences, and some moving birefringences. The SMs progressively increased into laminary layers after 43-days culture. The SMs, secreted by the epithelial cells in vitro, were very similar with the nacrum of the pearls and pearl shells. The contents of SMs during culture were detected by X-ray microanalyser. The SMs contained 59.43% Ca, 33.03% S and 1.6% P on the 10th day culture. Ca element in the SMs increased significantly on the 20th and 34th day culture, 71.86% and 92.03% respectively. At 43th day culture, containing 44.25% Ca, 4.98% S and 14.61% P were examined in the SMs. Other elelments, such as Si, K and Cl were also detected in the SMs，but containing little. The SMs were formed laminary layers made by CaCO3 crystals under the scanning electron microscope, and sticked by organic membrane. Because of the primary nacrum with large amounts of sulfur, it was related to nucleation and growth of CaCO3 crystals. This researching could provide help in culturing pearls in vitro or tube-pearls.
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Received: 01 January 1900
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