|
|
|
Study on Expressing A Segment of Infectious Bursal
Deasease Virus to Milk in Transgenic Mice |
| Lin Aixing Zhou Yun Hu Hongyu Hou Jian An Xiaorong Chen Yongfu** |
| （Sate Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094,China） |
|
|
|
|
Abstract Abstract The A segment of infectious bursal desease virus (IBDV) was ligated to the mammary gland expression vector p7GMB53. This transgenic construct was linearized to perform transgenic experiments, total 879 fertilized eggs were microinjected and transferred into 26 recipient mice, 39 mice were born with 4.4%(39/879) embryo survival rate. Results of PCR showed that 4 of 39 were positive. Southern hybridization showed 2 of 4 were positive, in which the copy numbers of IBDV integrated were 3(No.8) and 9(No.3) respectively. The female mouse No.3 was mated with nontransgenic male mouse and transgenic male mouse No.8. The ratio of positive mice was 52% (10/19) and 100%(5/5) respectively and the result indicated that the No.3 was a germ pure line. The No.8 was mated with nontransgenic female mouse and the ratio of positive mice was 11%(2/18), indicated the No.8 was a germ chimera. The milks of No.3 (female), No.6(the female offspring of No.3 and a nontransgenic mouse) and No.10 (the female offspring of the No.3 and No.8) were performed by Western blot and ELISA. Both of the mice expressed the foreign gene in mammary glands. We concluded preliminarily that this vector, containing matrix attachment region seguence(MARs), could drive foreign gene to express to milk independent effects of position in transgenic animals.
|
|
Received: 01 January 1900
|
|
Corresponding Authors:
Chen Yongfu**
|
|
|
|
|
|
|
