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Difference of Nuclear and Cytoplasmic in Vitro Maturation of
Mammalian Oocytes and Related Important Factors |
| Li Yonghai Liu Ruihua Jiao Lihong Wang Hong Wang Weihua** |
| (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China) |
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Abstract Abstract: Techniques of oocyte maturation in vitro affect the developmental competence of oocyte fertilization in vitro and embryo culture in vitro directly. It was reported that the requested environment or conditions were different between nuclear and cytoplasmic maturation in vitro. The criterion of nuclear maturation was indicated by the extrusion of first polar body and the oocyte reaching to metaphase Ⅱstage but there was no accepted standard for evaluating cytoplasmic maturation so far. Most of the researchers worldwide took the developmental competence of oocyte as their cytoplasmic maturation evaluating system. In general, cytoplasmic maturation of oocyte needs better conditions and longer culturing time than that of nuclear maturation in vitro. The conditions and results of oocyte maturation in vivo and in vitro, animals treated with hormones and some other factors affecting oocyte maturationin vitro and development in recent reports were discussed in the paper.
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Received: 01 January 1900
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