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| Molecular Identification of the Mutant Isolates of Magnaporthe grisea |
| He Yueqiu1,2 Hei LEUNG2 Robert S. ZEIGLER2 Tang Wenhua3 |
(1. Plant Protection College, Yunnan Agricultural University, Kunming 650201；
2. Entomology & Plant Pathology Division, International Rice Research Institute, Makati 3127, Philippines；
3. Plant Protection College, China Agricultural University, Beijing 100094, China) |
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Abstract Abstract: Pathogenic mutant isolates 9 from the greenhouse and 5 from the International Rice Research Institute Blast Nursery, respectively, were identified by using molecular techniques with Rep-PCR of the two primer pairs, Pot2-1/Pot2-2 and PMC1-2/PMC1-3, RAPD of 177 primers and RFLP of MGR586. The results indicated that the 9 mutants from greenhouse had almost identical DNA band patterns and their original isolates lost 1~3 bands for 7 mutants, but no changes for the other 2 mutants. The 5 mutants from the nursery, which defeated resistance genes Pi-1 and Pi-2, originated from the isolates with avirulence gene Avr-1 in the field, not from outside by genetic shift based on their pathotypes and DNA band patterns. Therefore, it was concluded that the mutation was a main evolution force for Magnaporthe grisea.
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Received: 01 January 1900
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