Introducing Glucose Oxidase Gene into Rice Mediated by Agrobacterium tumefaciens
Peng Hao1 Wang Zhixing2 Dou Daolong1 Zhu Shengwei 1 Lu Tiegang1 Sun Jingsan1*
(1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 2 .Biotechnology research institnte, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
Abstract Abstract: The glucose oxidase (GO ) gene with the function of broad-spectrum pathogen defense was inserted into a binary vector pCAMBIA1301 containing selectable marker of hygromycin resistant gene to produce a new expression vector, pCAG1301. The vector was introduced into Agrobacterium tumefaciens strain LBA4404 and then transformed them to immature embryos of rice (Oryza sativa L.ssp. japonica ) cultivar Nipponbare. Putative transformed plants were regenerated from hygromycin resistant calli, and the integration of GO gene was confirmed by Southern blotting analysis on rice genome. The H2O2 generated by the transgenic plants was detected by color reaction with starch-KI and this proved that the glucose oxidase generated by the expression of GO gene had exerted its function in rice. The transgenic plants were proved to have strong resistance to rice blast disease(Magnaporthe grisea ).
