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Introducing Glucose Oxidase Gene into Rice Mediated by
Agrobacterium tumefaciens
Peng Hao1 Wang Zhixing2 Dou Daolong1 Zhu Shengwei 1 Lu Tiegang1 Sun Jingsan1*
 (1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
 2 .Biotechnology research institnte, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
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Abstract     Abstract: The glucose oxidase (GO ) gene with the function of broad-spectrum pathogen defense was inserted into a binary vector pCAMBIA1301 containing selectable marker of hygromycin resistant gene to produce a new expression vector, pCAG1301.  The vector was introduced into Agrobacterium  tumefaciens strain LBA4404 and then transformed them to immature embryos of rice (Oryza sativa L.ssp. japonica ) cultivar Nipponbare. Putative transformed plants were regenerated from hygromycin resistant calli,  and the integration of GO gene was confirmed by Southern blotting analysis on rice genome. The H2O2 generated by the transgenic plants was detected by color reaction with starch-KI and this proved that the glucose oxidase generated by the expression of GO gene had exerted its function in rice. The transgenic plants were proved to have strong resistance to rice blast disease(Magnaporthe grisea ).
Key words Key words: rice      glucose oxidase      Agrobacterium tumefaciens       immature embryo transformation      hydrogen peroxide      rice blast disease     
Received: 01 January 1900     
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Peng Hao
Wang Zhixing
Dou Daolong
Zhu Shengwei
Lu Tiegang
Sun Jingsan
*
Cite this article:   
Peng Hao,Wang Zhixing,Dou Daolong, et al. Introducing Glucose Oxidase Gene into Rice Mediated by
Agrobacterium tumefaciens
[J]. , 2003, 11(1): 16-19.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2003/V11/I1/16
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