Analysis of Differentially Expressed Transcription Factor Genes in Sesame (Sesamum indicum) Under Low Nitrogen Stress
ZHANG Peng-Yu, SU Xiao-Yu, GAO Tong-Mei, LI Feng, TIAN Yuan, WANG Dong-Yong, WEI Shuang-Ling*
Sesame Research Center, Henan Academy of Agricultural Sciences/Henan Key Laboratory of Specific Oilseed Crops Genomics/Key Laboratory of Oil Crops in HuangHuaiHai Plains, Ministry of Agriculture and Rural Affairs, Zhengzhou 450002, China
Abstract Transcription factor families play important roles in plant response to nitrogen stress. In order to reveal the expression pattern of transcriptome factor genes in the sesame (Sesamum indicum) under low nitrogen stress, high-throughput sequencing technology was used to analyze the gene expression profile of transcription factors in the roots of sesame varieties 'Zhengzhi HL05' (ZZ, nitrogen-tolerance) and 'Myanmar high-yielding' (MD, nitrogen-sensitive) with different nitrogen efficiency under low nitrogen stress for 3 and 9 d. The results showed that a total of 55 transcription factor families were detected by transcriptome sequencing, with a total of 1 662 transcription factor genes, and 275 differentially expressed genes, accounting for 16.55% of the total. The families with a large number of differentially expressed transcription factor genes including MYB (avian myeloblastosis viral oncogenehomolog), bHLH (basic helix-loop-helix), ERF (ethylene responsive factor), NAC (NAM, ATAF1/2, CUC1/2) and WRKY families. Compared with the control, under low nitrogen stress for 3 d, 86 and 74 differentially expressed transcription factor genes were detected in 'Myanmar high-yielding' and 'Zhengzhi HL05' cultivars, respectively, and the number of down-regulated genes was more than that of up-regulated genes. Under low nitrogen stress for 9 d, 178 and 128 differentially expressed transcription factor genes were detected in each cultivar, and the number of up-regulated genes was more than or equal to that of down-regulated genes. The transcription factor genes in response to low nitrogen stress showed cultivar- specificity. There were 113 and 54 transcription factor genes specifically expressed in 'Myanmar high-yielding' and 'Zhengzhi HL05', respectively. The Venn diagram of differentially expressed transcription factor genes showed that a total of 15 transcription factor genes were differentially expressed in different treatment groups, of which 8 genes up-regulated and 7 genes down-regulated. The results of qRT-PCR validation showed that the change trend of 15 differentially expressed genes was consistent with the sequencing results, which proved the validity of the sequencing results. This study provides a reference basis for further exploring the molecular response mechanism of sesame transcription factor family to low nitrogen stress.
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