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| Cloning and Expression Analysis of Linalool Synthase CcTPS14 from Cinnamomum camphora var. linaloolifera |
| CAO Rui-Lan1, ZHOU Zeng-Liang2, REN Zhi-Hua1, WANG Xi-Zheng1, CHENG Qin-Hua1, HU Dong-Nan1, CHEN Shang-Xing1, LIU Juan1* |
1 Jiangxi Provincial Key Laboratory of Silviculture/College of Forestry, Jiangxi Agricultural University, Nanchang 330045;
2 Jiangxi Forestry Ecological Engineering Construction Center, Nanchang 330103 |
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Abstract Cinnamomum camphora var. linaloolifera is a kind of camphor trees with linalool-rich essential oils in their branches and leaves. Due to its high economic value, this variety has become one of the important industrial material tree species in South China. In this study, a linalool synthase gene (CcTPS14) was cloned from C. camphora var. linaloolifera, and was analyzed based on bioinformatics, phylogeny and tissue-specific expression patterns. The results showed that the full length of CcTPS14 was 725 bp, with a 684 bp CDS and encoding 227 amino acids. The relative molecular weight of CcTPS14 protein was 25.69 kD, the theoretical isoelectric point was 5.39. The fat coefficient was 87.19 and the instability coefficient was 51.40. The CcTPS14 protein was unstable and hydrophilic. Based on subcellular localization predicts, CcTPS14 was mostly located in chloroplasts. Its secondary structure was mainly irregular crimping (65.86%) and α -helix (22.52%), with a small amount of extended chains (12.60%) and β-folding (9.01%). Phylogenetic relationship analysis showed that CcTPS14 was closely related to TPS from C. micranthum and C. osmophloeum, indicating that the presence of similar evolutionary process and biological function of those TPS genes in different species. And it was distantly related to TPS genes from Tetracentron sinensis and Rhododendron griersonianum. The relative expression of CcTPS14 in different tissues of C. camphora was significantly different (P<0.05), among which the expression was higher in mature tissues (such as stems and old leaves) and lower in young tissues (such as young leaves, young fruits and flowers). This study provides the molecular basis and theoretical reference for further understanding of the functional of CcTPS14.
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Received: 28 March 2022
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Corresponding Authors:
* liu_juan1122@163.com
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